Systematic phenotypic analysis

I. Systematic phenotypic analysis of C. elegans lineage differentiation over time, space and genome

  • RNAi screening of 1,061 embryonic essential genes identified 204 developmental regulatory genes with broad molecular and cellular functions
  • Three tissue-specific markers were used to assay lineage differentiation
  • Systematic lineage tracing and marker expression mapping for 1,368 embryos with embryonic lethal phenotypes

II. Systematic Perturbation of Lineage Differentiation

  • 4,657 clonal fate changes
  • 201 genes induced 820 cell fate changes in progenior cells

III. Trajectories and Regulators of Cell Fate Choice During Lineage Differentiation

IV. A Multiscale Model of Lineage Differentiation