Introduction
Systematic phenotypic analysis
I. Systematic phenotypic analysis of C. elegans lineage differentiation over time, space and genome
- RNAi screening of 1,061 embryonic essential genes identified 204 developmental regulatory genes with broad molecular and cellular functions
- Three tissue-specific markers were used to assay lineage differentiation
- Systematic lineage tracing and marker expression mapping for 1,368 embryos with embryonic lethal phenotypes
II. Systematic Perturbation of Lineage Differentiation
- 4,657 clonal fate changes
- 201 genes induced 820 cell fate changes in progenior cells
III. Trajectories and Regulators of Cell Fate Choice During Lineage Differentiation
- 76 genes regulate 32 types of cell fate choices for 175 events
- Inferred trajectories of lineage differentiation
IV. A Multiscale Model of Lineage Differentiation
- Global gene regulatory network with 194 gene nodes and 2,689 edges
- A multiscale model that integrates 25 progenitor cell fates, 56 fate trajectories and 52 cell-specific gene networks
